I’m so sorry to hear that you’ve hit this snag! I appreciate you taking the time to highlight the area you noticed the trouble occurring, and for sending over all of these great details. I’ve extracted the log files from your Glowforge to review this particular print, and compare it with the information you’ve provided.
I see that you’ve recently performed a camera calibration, which may help to ensure the alignment does not run into the same trouble. Have you been able to perform some additional prints after the calibration to see if the same error occurs in this spot in your Glowforge’s bed?
I do see that the material may be positioned a little too far towards the bottom of the crumb tray, but I understand that you are also attempting to utilize your Passthrough slot. Have you noticed this behavior while utilizing the Passthrough software?
Let us know!