The head will be able to to go over it, but the air assist will hit it - so if you’re only engraving along the top edge - and you’ll survive if it bumps and fails - totes worth a shot. If you’re trying to engrave in the middle, it’ll definitely fail.
Honestly though, I avoided removing the tray forever cuz it was scary, but with set focus I literally just guesstimate and pile the thing on top of a few random pieces of wood and if the measurement fails I add another piece of wood. You’re trying to get the thing so it’s ~1/2" away from the laser. There are things you can cut which make it brainless, but I’m lazy that way 