Hello and, welcome to the forum!
Were you getting better alignment on previous jobs before this one? If so, is there any possibility you bumped the head or arm of the machine after you turned it on and, it had centered? That can cause alignment to be off until the machine is next power cycled.
You may need to run the alignment again if you physically moved your machine but, it should not otherwise suddenly change.
As for the label: yes, the label adds additional material between the laser and the board. So, it can reduce the amount of energy getting to the board and, interfere with the engrave. Some folks here even remove all of the masking when engraving for that reason.
Posting in Problems & Support opens a support ticket with Glowforge. So, you’ll likely hear from them within a couple days. Some of the other forum members might have other suggestions before then.