Everything that @evansd2 wrote, but also, how thick is your material (it looks weirdly tall in the UI photo)? It is taller than half an inch? If it’s too tall, that’ll throw things off too. You’ve had it since 2021, so you probably know this already.
I always make a cardboard jig when i need perfect alignment, but I also try to place all my objects as close to the center under the camera as possible so that it’s most accurate. It tends to get distorted near the edges, like a fisheye view. There is a thread on this forum where a user shared a how to on using a grid she made for perfect alignment without using the camera, you might want to use that if the objects needs to be apart like that.
If it’s really off after you use set focus (I’d probably test with a light, quick score on cardboard first), then you might need to do a camera calibration, but usually that only needs to be done after you move the machine.